2025

Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination.

Shin Y, Petassi MT, Jessop AM, Kim SY, Matei R, Morse K, Raina VB, Roy U, Greene EC.

bioRxiv [Preprint]. 2025 Mar 26:2025.03.26.645561. doi: 10.1101/2025.03.26.645561. PMID: 40196570

ATP hydrolysis-driven structural transitions within the S. cerevisiae Rad51 and Dmc1 nucleoprotein filaments.

Shin Y, Kim SY, Greene EC.

bioRxiv [Preprint]. 2025 Mar 19:2025.03.19.644215. doi: 10.1101/2025.03.19.644215. PMID: 40166259

Biochemical Mechanisms of Genetic Recombination and DNA Repair.

Raina VB, Jessop A, Greene EC.

Annu Rev Biochem. 2025 Mar 28. doi: 10.1146/annurev-biochem-083024-113931. Online ahead of print. PMID: 40153609

Single-Molecule Visualization of BLM-DNA2-Mediated DNA End Resection Using DNA Curtains.

Raina VB, Greene EC.

Methods Mol Biol. 2025;2881:195-211. doi: 10.1007/978-1-0716-4280-1_10. PMID: 39704945

2024

Lineage-specific amino acids define functional attributes of the protomer-protomer interfaces for the Rad51 and Dmc1 recombinases.

Petassi M, Shin Y, Jessop AM, Morse K, Kim SY, Matei R, Raina VB, Greene EC.

bioRxiv [Preprint]. 2024 Dec 4:2024.12.03.626531. doi: 10.1101/2024.12.03.626531.

PMID: 39677717

Promotion of DNA end resection by BRCA1-BARD1 in homologous recombination.

Salunkhe S, Daley JM, Kaur H, Tomimatsu N, Xue C, Raina VB, Jasper AM, Rogers CM, Li W, Zhou S, Mojidra R, Kwon Y, Fang Q, Ji JH, Badamchi Shabestari A, Fitzgerald O, Dinh H, Mukherjee B, Habib AA, Hromas R, Mazin AV, Wasmuth EV, Olsen SK, Libich DS, Zhou D, Zhao W, Greene EC, Burma S, Sung P.

Nature. 2024 Oct;634(8033):482-491. doi: 10.1038/s41586-024-07910-2. Epub 2024 Sep 11.

PMID: 39261729

2023

The separation pin distinguishes the pro- and anti-recombinogenic functions of Saccharomyces cerevisiae Srs2.

Meir A, Raina VB, Rivera CE, Marie L, Symington LS, Greene EC.

Nat Commun. 2023 Dec 8;14(1):8144. doi: 10.1038/s41467-023-43918-4.

PMID: 38065943

Mechanism of target site selection by type V-K CRISPR-associated transposases.

George JT, Acree C, Park JU, Kong M, Wiegand T, Pignot YL, Kellogg EH, Greene EC, Sternberg SH.

Science. 2023 Nov 17;382(6672):eadj8543. doi: 10.1126/science.adj8543. Epub 2023 Nov 17.

PMID: 37972161

Structural insights into BCDX2 complex function in homologous recombination.

Rawal Y, Jia L, Meir A, Zhou S, Kaur H, Ruben EA, Kwon Y, Bernstein KA, Jasin M, Taylor AB, Burma S, Hromas R, Mazin AV, Zhao W, Zhou D, Wasmuth EV, Greene EC, Sung P, Olsen SK.

Nature. 2023 Jul;619(7970):640-649. doi: 10.1038/s41586-023-06219-w. Epub 2023 Jun 21.

PMID: 37344589

Single-molecule visualization of Pif1 helicase translocation on single-stranded DNA.

Mustafi M, Kwon Y, Sung P, Greene EC.

J Biol Chem. 2023 Jun;299(6):104817. doi: 10.1016/j.jbc.2023.104817. Epub 2023 May 11.

PMID: 37178921

2022

Repair of mismatched templates during Rad51-dependent Break-Induced Replication.

Choi J, Kong M, Gallagher DN, Li K, Bronk G, Cao Y, Greene EC, Haber JE.

PLoS Genet. 2022 Sep 2;18(9):e1010056. doi: 10.1371/journal.pgen.1010056. eCollection 2022 Sep.

PMID: 36054210

Bloom helicase mediates formation of large single-stranded DNA loops during DNA end processing.

Xue C, Salunkhe SJ, Tomimatsu N, Kawale AS, Kwon Y, Burma S, Sung P, Greene EC.

Nat Commun. 2022 Apr 26;13(1):2248. doi: 10.1038/s41467-022-29937-7.

PMID: 35473934

Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.

Meir A, Crickard JB, Kwon Y, Sung P, Greene EC.

Proc Natl Acad Sci U S A. 2022 Jan 25;119(4):e2113871119. doi: 10.1073/pnas.2113871119.

PMID: 35042797

Structure-activity relationships at a nucleobase-stacking tryptophan required for chemomechanical coupling in the DNA resecting motor-nuclease AdnAB.

Warren GM, Meir A, Wang J, Patel DJ, Greene EC, Shuman S.

Nucleic Acids Res. 2022 Jan 25;50(2):952-961. doi: 10.1093/nar/gkab1270.

PMID: 34967418

2021

Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks.

Kong M, Greene EC.

Front Cell Dev Biol. 2021 Nov 15;9:745311. doi: 10.3389/fcell.2021.745311. eCollection 2021.

PMID: 34869333

Single-molecule studies of yeast Rad51 paralogs.

Roy U, Kwon Y, Sung P, Greene EC.

Methods Enzymol. 2021;661:343-362. doi: 10.1016/bs.mie.2021.08.006. Epub 2021 Sep 10.

PMID: 34776219

Editorial overview: Recombination - the ends justify the means.

Greene EC, Rothstein R.

Curr Opin Genet Dev. 2021 Dec;71:iii-vii. doi: 10.1016/j.gde.2021.10.003. Epub 2021 Nov 8.

PMID: 34764004

Computed structures of core eukaryotic protein complexes.

Humphreys IR, Pei J, Baek M, Krishnakumar A, Anishchenko I, Ovchinnikov S, Zhang J, Ness TJ, Banjade S, Bagde SR, Stancheva VG, Li XH, Liu K, Zheng Z, Barrero DJ, Roy U, Kuper J, Fernández IS, Szakal B, Branzei D, Rizo J, Kisker C, Greene EC, Biggins S, Keeney S, Miller EA, Fromme JC, Hendrickson TL, Cong Q, Baker D.

Science. 2021 Dec 10;374(6573):eabm4805. doi: 10.1126/science.abm4805. Epub 2021 Dec 10.

PMID: 34762488

The Role of the Rad55-Rad57 Complex in DNA Repair.

Roy U, Greene EC.

Genes (Basel). 2021 Sep 8;12(9):1390. doi: 10.3390/genes12091390.

PMID: 34573372

Srs2 and Pif1 as Model Systems for Understanding Sf1a and Sf1b Helicase Structure and Function.

Meir A, Greene EC.

Genes (Basel). 2021 Aug 26;12(9):1319. doi: 10.3390/genes12091319.

PMID: 34573298

DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing.

Xue C, Greene EC.

Trends Genet. 2021 Jul;37(7):639-656. doi: 10.1016/j.tig.2021.02.008. Epub 2021 Apr 22.

PMID: 33896583

Single-Stranded DNA Curtains for Single-Molecule Visualization of Rad51-ssDNA Filament Dynamics.

Roy U, Greene EC.

Methods Mol Biol. 2021;2281:193-207. doi: 10.1007/978-1-0716-1290-3_11.

PMID: 33847959

Clutch mechanism of chemomechanical coupling in a DNA resecting motor nuclease.

Unciuleac MC, Meir A, Xue C, Warren GM, Greene EC, Shuman S.

Proc Natl Acad Sci U S A. 2021 Mar 16;118(11):e2023955118. doi: 10.1073/pnas.2023955118.

PMID: 33836607

RADX controls RAD51 filament dynamics to regulate replication fork stability.

Adolph MB, Mohamed TM, Balakrishnan S, Xue C, Morati F, Modesti M, Greene EC, Chazin WJ, Cortez D.

Mol Cell. 2021 Mar 4;81(5):1074-1083.e5. doi: 10.1016/j.molcel.2020.12.036. Epub 2021 Jan 15.

PMID: 33453169

The Rad51 paralog complex Rad55-Rad57 acts as a molecular chaperone during homologous recombination.

Roy U, Kwon Y, Marie L, Symington L, Sung P, Lisby M, Greene EC.

Mol Cell. 2021 Mar 4;81(5):1043-1057.e8. doi: 10.1016/j.molcel.2020.12.019. Epub 2021 Jan 8.

PMID: 33421364

Single-molecule visualization of human RECQ5 interactions with single-stranded DNA recombination intermediates.

Xue C, Molnarova L, Steinfeld JB, Zhao W, Ma C, Spirek M, Kaniecki K, Kwon Y, Beláň O, Krejci K, Boulton SJ, Sung P, Greene EC, Krejci L.

Nucleic Acids Res. 2021 Jan 11;49(1):285-305. doi: 10.1093/nar/gkaa1184.

PMID: 33332547

2020

Demystifying the D-loop during DNA recombination.

Roy U, Greene EC.

Nature. 2020 Oct;586(7831):677-678. doi: 10.1038/d41586-020-02831-2.

PMID: 33057188

Rad54 and Rdh54 occupy spatially and functionally distinct sites within the Rad51-ssDNA presynaptic complex.

Crickard JB, Kwon Y, Sung P, Greene EC.

EMBO J. 2020 Oct 15;39(20):e105705. doi: 10.15252/embj.2020105705. Epub 2020 Aug 13.

PMID: 32790929

DNA Curtains Shed Light on Complex Molecular Systems During Homologous Recombination.

Meir A, Kong M, Xue C, Greene EC.

J Vis Exp. 2020 Jun 23;(160). doi: 10.3791/61320.

PMID: 32658186

Rad54 Drives ATP Hydrolysis-Dependent DNA Sequence Alignment during Homologous Recombination.

Crickard JB, Moevus CJ, Kwon Y, Sung P, Greene EC.

Cell. 2020 Jun 11;181(6):1380-1394.e18. doi: 10.1016/j.cell.2020.04.056. Epub 2020 Jun 4.

PMID: 32502392

Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA.

Kong M, Cutts EE, Pan D, Beuron F, Kaliyappan T, Xue C, Morris EP, Musacchio A, Vannini A, Greene EC.

Mol Cell. 2020 Jul 2;79(1):99-114.e9. doi: 10.1016/j.molcel.2020.04.026. Epub 2020 May 22.

PMID: 32445620