Defining the influence of Rad51 and Dmc1 lineage-specific amino acids on genetic recombination.

Steinfeld JB, Beláň O, Kwon Y, Terakawa T, Al-Zain A, Smith MJ, Crickard JB, Qi Z, Zhao W, Rothstein R, Symington LS, Sung P, Boulton SJ, Greene EC.

Genes Dev. 2019 Aug 1. doi: 10.1101/gad.328062.119. [Epub ahead of print]

PMID: 31371435

The RecQ helicase Sgs1 drives ATP-dependent disruption of Rad51 filaments.

Crickard JB, Xue C, Wang W, Kwon Y, Sung P, Greene EC.

Nucleic Acids Res. 2019 May 21;47(9):4694-4706. doi: 10.1093/nar/gkz186.

PMID: 30916344

Helicase Mechanisms During Homologous Recombination in Saccharomyces cerevisiae.

Crickard JB, Greene EC.

Annu Rev Biophys. 2019 Mar 11. doi: 10.1146/annurev-biophys-052118-115418. [Epub ahead of print]

PMID: 30857400

Regulatory control of Sgs1 and Dna2 during eukaryotic DNA end resection.

Xue C, Wang W, Crickard JB, Moevus CJ, Kwon Y, Sung P, Greene EC.

Proc Natl Acad Sci U S A. 2019 Mar 26;116(13):6091-6100. doi: 10.1073/pnas.1819276116. Epub 2019 Mar 8.

PMID: 30850524

Dynamic interactions of the homologous pairing 2 (Hop2)-meiotic nuclear divisions 1 (Mnd1) protein complex with meiotic presynaptic filaments in budding yeast.

Crickard JB, Kwon Y, Sung P, Greene EC.

J Biol Chem. 2019 Jan 11;294(2):490-501. doi: 10.1074/jbc.RA118.006146. Epub 2018 Nov 12.

PMID: 30420424


The biochemistry of early meiotic recombination intermediates.

Crickard JB, Greene EC.

Cell Cycle. 2018;17(23):2520-2530. doi: 10.1080/15384101.2018.1553355. Epub 2018 Dec 10.

PMID: 30482074

New roles for RAD52 in DNA repair.

Xue C, Greene EC.

Cell Res. 2018 Dec;28(12):1127-1128. doi: 10.1038/s41422-018-0105-8. No abstract available.

PMID: 30367126

Meiosis-specific recombinase Dmc1 is a potent inhibitor of the Srs2 antirecombinase.

Crickard JB, Kaniecki K, Kwon Y, Sung P, Greene EC.

Proc Natl Acad Sci U S A. 2018 Oct 9. pii: 201810457. doi: 10.1073/pnas.1810457115. [Epub ahead of print]

PMID: 30301803

Biochemical attributes of mitotic and meiotic presynaptic complexes.

Crickard JB, Greene EC.

DNA Repair (Amst). 2018 Nov;71:148-157. doi: 10.1016/j.dnarep.2018.08.018. Epub 2018 Aug 23. Review.

PMID: 30195641

Single-Stranded DNA Curtains for Studying the Srs2 Helicase Using Total Internal Reflection Fluorescence Microscopy.

De Tullio L, Kaniecki K, Greene EC.

Methods Enzymol. 2018;600:407-437. doi: 10.1016/bs.mie.2017.12.004. Epub 2018 Feb 1.

PMID: 29458768

Regulation of Hed1 and Rad54 binding during maturation of the meiosis-specific presynaptic complex.

Crickard JB, Kaniecki K, Kwon Y, Sung P, Lisby M, Greene EC.

EMBO J. 2018 Apr 3;37(7). pii: e98728. doi: 10.15252/embj.201798728. Epub 2018 Feb 14.

PMID: 29444896


Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments.

Crickard JB, Kaniecki K, Kwon Y, Sung P, Greene EC.

J Biol Chem. 2018 Mar 16;293(11):4191-4200. doi: 10.1074/jbc.RA117.001143. Epub 2018 Jan 30.

PMID: 29382724

A change of view: homologous recombination at single-molecule resolution.

Kaniecki K, De Tullio L, Greene EC.

Nat Rev Genet. 2018 Apr;19(4):191-207. doi: 10.1038/nrg.2017.92. Epub 2017 Dec 11. Review.

PMID: 29225334



Dissociation of Rad51 Presynaptic Complexes and Heteroduplex DNA Joints by Tandem Assemblies of Srs2.

Kaniecki K, De Tullio L, Gibb B, Kwon Y, Sung P, Greene EC.

Cell Rep. 2017 Dec 12;21(11):3166-3177. doi: 10.1016/j.celrep.2017.11.047.

PMID: 29241544


The condensin complex is a mechanochemical motor that translocates along DNA.

Terakawa T, Bisht S, Eeftens JM, Dekker C, Haering CH, Greene EC.

Science. 2017 Nov 3;358(6363):672-676. doi: 10.1126/science.aan6516. Epub 2017 Sep 7.

PMID: 28882993


BRCA1-BARD1 promotes RAD51-mediated homologous DNA pairing.

Zhao W, Steinfeld JB, Liang F, Chen X, Maranon DG, Jian Ma C, Kwon Y, Rao T, Wang W, Sheng C, Song X, Deng Y, Jimenez-Sainz J, Lu L, Jensen RB, Xiong Y, Kupfer GM, Wiese C, Greene EC, Sung P.

Nature. 2017 Oct 19;550(7676):360-365. doi: 10.1038/nature24060. Epub 2017 Oct 4.

PMID: 28976962


Yeast Srs2 Helicase Promotes Redistribution of Single-Stranded DNA-Bound RPA and Rad52 in Homologous Recombination Regulation.

De Tullio L, Kaniecki K, Kwon Y, Crickard JB, Sung P, Greene EC.

Cell Rep. 2017 Oct 17;21(3):570-577. doi: 10.1016/j.celrep.2017.09.073.

PMID: 29045827


Monitoring Replication Protein A (RPA) dynamics in homologous recombination through site-specific incorporation of non-canonical amino acids.

Pokhrel N, Origanti S, Davenport EP, Gandhi D, Kaniecki K, Mehl RA, Greene EC, Dockendorff C, Antony E.

Nucleic Acids Res. 2017 Sep 19;45(16):9413-9426. doi: 10.1093/nar/gkx598.

PMID: 28934470


Sequential eviction of crowded nucleoprotein complexes by the exonuclease RecBCD molecular motor.

Terakawa T, Redding S, Silverstein TD, Greene EC.

Proc Natl Acad Sci U S A. 2017 Aug 1;114(31):E6322-E6331. doi: 10.1073/pnas.1701368114. Epub 2017 Jul 17.

PMID: 28716908


Human RAD52 interactions with replication protein A and the RAD51 presynaptic complex.

Ma CJ, Kwon Y, Sung P, Greene EC.

J Biol Chem. 2017 Jul 14;292(28):11702-11713. doi: 10.1074/jbc.M117.794545. Epub 2017 May 27.

PMID: 28551686


Sequence imperfections and base triplet recognition by the Rad51/RecA family of recombinases.

Lee JY, Steinfeld JB, Qi Z, Kwon Y, Sung P, Greene EC.

J Biol Chem. 2017 Jun 30;292(26):11125-11135. doi: 10.1074/jbc.M117.787614. Epub 2017 May 5.

PMID: 28476890


Replication Protein A Blocks the Way.

Greene EC.

Biochemistry. 2017 Apr 4;56(13):1809-1810. doi: 10.1021/acs.biochem.7b00221. Epub 2017 Mar 23. No abstract available.

PMID: 28333436


Protein dynamics of human RPA and RAD51 on ssDNA during assembly and disassembly of the RAD51 filament.

Ma CJ, Gibb B, Kwon Y, Sung P, Greene EC.

Nucleic Acids Res. 2017 Jan 25;45(2):749-761. doi: 10.1093/nar/gkw1125. Epub 2016 Nov 29.

PMID: 27903895


Telomere Recognition and Assembly Mechanism of Mammalian Shelterin.

Erdel F, Kratz K, Willcox S, Griffith JD, Greene EC, de Lange T.

Cell Rep. 2017 Jan 3;18(1):41-53. doi: 10.1016/j.celrep.2016.12.005.

PMID: 28052260


Single-Stranded DNA Curtains for Studying Homologous Recombination.

Ma CJ, Steinfeld JB, Greene EC.

Methods Enzymol. 2017;582:193-219. doi: 10.1016/bs.mie.2016.08.005. Epub 2016 Oct 22.

PMID: 28062035


A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling.

Taylor MRG, Špírek M, Jian Ma C, Carzaniga R, Takaki T, Collinson LM, Greene EC, Krejci L, Boulton SJ.

Mol Cell. 2016 Dec 1;64(5):926-939. doi: 10.1016/j.molcel.2016.10.020. Epub 2016 Nov 17.

PMID: 27867009


ATP hydrolysis Promotes Duplex DNA Release by the RecA Presynaptic Complex.

Lee JY, Qi Z, Greene EC.

J Biol Chem. 2016 Oct 14;291(42):22218-22230. Epub 2016 Sep 1.

PMID: 27587394


Visualizing recombination intermediates with single-stranded DNA curtains.

Qi Z, Greene EC.

Methods. 2016 Aug 1;105:62-74. doi: 10.1016/j.ymeth.2016.03.027. Epub 2016 Mar 30. Review.

PMID: 27038747


Generalized nucleation and looping model for epigenetic memory of histone modifications.

Erdel F, Greene EC.

Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):E4180-9. doi: 10.1073/pnas.1605862113. Epub 2016 Jul 5.

PMID: 27382173

DNA Sequence Alignment during Homologous Recombination.

Greene EC.

J Biol Chem. 2016 May 27;291(22):11572-80. doi: 10.1074/jbc.R116.724807. Epub 2016 Apr 15. Review.

PMID: 27129270

Single-Molecule Imaging Reveals a Collapsed Conformational State for DNA-Bound Cohesin.

Stigler J, Çamdere GÖ, Koshland DE, Greene EC.

Cell Rep. 2016 May 3;15(5):988-98. doi: 10.1016/j.celrep.2016.04.003. Epub 2016 Apr 21.



On the influence of protein-DNA register during homologous recombination.

Greene EC.

Cell Cycle. 2016;15(2):172-5. doi: 10.1080/15384101.2015.1121352.

PMID: 26652653


Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System.

Redding S, Sternberg SH, Marshall M, Gibb B, Bhat P, Guegler CK, Wiedenheft B, Doudna JA, Greene EC.

Cell. 2015 Nov 5;163(4):854-65. doi: 10.1016/j.cell.2015.10.003.

PMID: 26522594


DNA RECOMBINATION. Base triplet stepping by the Rad51/RecA family of recombinases.

Lee JY, Terakawa T, Qi Z, Steinfeld JB, Redding S, Kwon Y, Gaines WA, Zhao W, Sung P, Greene EC.

Science. 2015 Aug 28;349(6251):977-81. doi: 10.1126/science.aab2666. Erratum in: Science. 2015 Oct 30;350(6260):aad6940.

PMID: 26315438


The Dynamics of Eukaryotic Replication Initiation: Origin Specificity, Licensing, and Firing at the Single-Molecule Level

Duzdevich D, Warner MD, Ticau S, Ivica NA, Bell SP,  Greene EC..

Mol Cell. 2015 May 7;58(3):483-94. doi: 10.1016/j.molcel.2015.03.017. Epub 2015 Apr 23.

PMID: 25921072


A Molecular Take on Aesop's The Oak and the Reeds.

Moevus CJ, Greene EC.

Cell. 2015 Mar 12;160(6):1039-40. doi: 10.1016/j.cell.2015.02.036.

PMID: 25768898


DNA Sequence Alignment by Microhomology Sampling during Homologous Recombination.

Qi Z, Redding S, Lee JY, Gibb B, Kwon Y, Niu H, Gaines WA, Sung P, Greene EC.

Cell. 2015 Feb 26;160(5):856-69. doi: 10.1016/j.cell.2015.01.029. Epub 2015 Feb 12.

PMID: 25684365


Protein dynamics during presynaptic-complex assembly on individual single-stranded DNA molecules.

Gibb B, Ye LF, Kwon Y, Niu H, Sung P, Greene EC.

Nat Struct Mol Biol. 2014 Oct;21(10):893-900. doi: 10.1038/nsmb.2886. Epub 2014 Sep 7.

PMID: 25195049


DNA curtains: novel tools for imaging protein-nucleic acid interactions at the single-molecule level.

Collins BE, Ye LF, Duzdevich D, Greene EC.

Methods Cell Biol. 2014;123:217-34. doi: 10.1016/B978-0-12-420138-5.00012-4. Review.

PMID: 24974030


Single-molecule imaging of FtsK translocation reveals mechanistic features of protein-protein collisions on DNA.

Lee JY, Finkelstein IJ, Arciszewska LK, Sherratt DJ, Greene EC.

Mol Cell. 2014 Jun 5;54(5):832-43. doi: 10.1016/j.molcel.2014.03.033. Epub 2014 Apr 24.

PMID: 24768536


RPA antagonizes microhomology-mediated repair of DNA double-strand breaks.

Deng SK, Gibb B, de Almeida MJ, Greene EC, Symington LS.

Nat Struct Mol Biol. 2014 Apr;21(4):405-12. doi: 10.1038/nsmb.2786. Epub 2014 Mar 9.

PMID: 24608368


Visualizing protein movement on DNA at the single-molecule level using DNA curtains.

Silverstein TD, Gibb B, Greene EC.

DNA Repair (Amst). 2014 Aug;20:94-109. doi: 10.1016/j.dnarep.2014.02.004. Epub 2014 Mar 2.

PMID: 24598576


Concentration-dependent exchange of replication protein A on single-stranded DNA revealed by single-molecule imaging.

Gibb B, Ye LF, Gergoudis SC, Kwon Y, Niu H, Sung P, Greene EC.

PLoS One. 2014 Feb 3;9(2):e87922. doi: 10.1371/journal.pone.0087922. eCollection 2014.

PMID: 24498402


DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.

Sternberg SH, Redding S, Jinek M, Greene EC, Doudna JA.

Nature. 2014 Mar 6;507(7490):62-7. doi: 10.1038/nature13011. Epub 2014 Jan 29.

PMID: 24476820


DNA dynamics and single-molecule biology.

Duzdevich D, Redding S, Greene EC.

Chem Rev. 2014 Mar 26;114(6):3072-86. doi: 10.1021/cr4004117. Epub 2014 Jan 8. Review. No abstract available. 

PMID: 24400809


How do proteins locate specific targets in DNA?

Redding S, Greene EC.

Chem Phys Lett. 2013 May 10;570. doi: 10.1016/j.cplett.2013.03.035.

PMID: 24187380


Tension modulates actin filament polymerization mediated by formin and profilin.

Courtemanche N, Lee JY, Pollard TD, Greene EC.

Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):9752-7. doi: 10.1073/pnas.1308257110. Epub 2013 May 28.

PMID: 23716666


Molecular traffic jams on DNA.

Finkelstein IJ, Greene EC.

Annu Rev Biophys. 2013;42:241-63. doi: 10.1146/annurev-biophys-083012-130304. Epub 2013 Feb 28. Review.

PMID: 23451891


Target search dynamics during post-replicative mismatch repair.

Gorman J, Greene EC.

Cell Cycle. 2013 Feb 15;12(4):537-8. doi: 10.4161/cc.23669. Epub 2013 Jan 23. No abstract available. 

PMID: 23343769


Towards physiological complexity with in vitro single-molecule biophysics.

Duzdevich D, Greene EC.

Philos Trans R Soc Lond B Biol Sci. 2012 Dec 24;368(1611):20120271. doi: 10.1098/rstb.2012.0271. Print 2013 Feb 5. Review.

PMID: 23267187


The promoter-search mechanism of Escherichia coli RNA polymerase is dominated by three-dimensional diffusion.

Wang F, Redding S, Finkelstein IJ, Gorman J, Reichman DR, Greene EC.

Nat Struct Mol Biol. 2013 Feb;20(2):174-81. doi: 10.1038/nsmb.2472. Epub 2012 Dec 23.

PMID: 23262491


Sliding to the rescue of damaged DNA.

Gibb B, Greene EC.

Elife. 2012 Dec 13;1:e00347. doi: 10.7554/eLife.00347.

PMID: 23240090